Xi'an Kintai Biotech Inc is one of the most experienced manufacturers and suppliers of sulforaphane powder in China. Please feel free to wholesale high quality sulforaphane powder for sale here from our factory. For price consultation, contact us.
sulforaphane powder supplier / manufacturer
KINTAIBIO® offers high-quality sulforaphane powder in 1%, 5%, 10% sulforaphane content specifications, and can provide customized product solutions according to customer needs. The product is primarily derived from high-quality broccoli seeds, prepared using modern plant extraction, separation, purification, and quality control technologies. It exhibits good stability and application value, and is widely used in functional foods, dietary supplements, nutritional health products, and scientific research and development.
Sulforaphane is a naturally occurring isothiocyanate active substance found in cruciferous plants and is considered one of the most valuable plant active ingredients in broccoli for research. It mainly exists in the precursor form of sulforaphane in plant tissues. When plant cells are damaged, sulforaphane is converted into bioactive sulforaphane under the action of myrosinase. Broccoli seeds, due to their high sulforaphane content, are currently an important natural source for extracting sulforaphane and related functional ingredients.If you require broccoli seed extract (Sulforaphane / Glucosinolate), please contact us at sales@kintaibio.com.
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COA Of sulforaphane powder
how to get sulforaphane?
Raw Material Selection & Grinding
Production uses high-quality dried broccoli seeds or fresh broccoli heads as the main raw materials. First, the raw materials are selected, cleaned, and pre-treated to remove impurities and ensure the quality meets production requirements. The treated broccoli raw materials are then crushed using a pulverizing device to achieve a particle size of 30-60 mesh. This effectively disrupts the plant cell structure, allowing for the full release of glucoraphane and related enzymes, thus improving the efficiency of subsequent enzymatic hydrolysis.
Enzymatic Hydrolysis
The crushed broccoli raw materials are mixed with 1.5-2 times their weight of pure water, and the pH of the system is adjusted to 4.1-4.5 using a 5% hydrochloric acid solution to establish an acidic environment suitable for the myrosinase reaction. Then, 1g of crude myrosinase (crude black myrosinase) is added per kilogram of raw materials, and the enzymatic hydrolysis reaction is carried out at 31-34℃ for 1-3 hours. Glucosamine undergoes hydrolysis under the action of myrosinase, transforming into active sulforaphane.
Freeze Drying and Material Stabilization
The broccoli solid-liquid mixture after enzymatic hydrolysis is freeze-dried to remove moisture at low temperatures, ensuring the final powder moisture content is below 5%. Freeze-drying effectively reduces the degradation of heat-sensitive active substances while improving material stability, providing suitable conditions for subsequent organic solvent extraction. The freeze-dried powder then proceeds to the next extraction step.
Solvent Extraction and Crude Product Preparation
The freeze-dried broccoli powder is extracted with pure acetone. Pure acetone is added three times by weight in three separate extractions at room temperature, with a total extraction time of 4–8 hours. After extraction, plant residues are separated by filtration, and the three acetone extracts are combined and concentrated under vacuum at 35–40°C to remove the solvent, yielding crude sulforaphane.
Multistage Column Chromatographic Purification
The obtained crude sulforaphane was further purified using silica gel column chromatography. During purification, a mixture of ethyl acetate and ethanol was used as the eluent, and the target components were separated by gradient elution. The fraction containing sulforaphane was collected and concentrated under vacuum at 35–40°C. Subsequently, the concentrated sulforaphane was dissolved in methanol, filtered through a 0.2 μm microporous membrane, and further refined using a 20–40 μm C18 silica gel column. Gradient elution was performed using methanol-water systems of different concentrations to remove impurities.
Vacuum Drying & Final Product
The sulforaphane fraction purified by the C18 column was collected and subjected to desolvation under vacuum drying at 35–40°C to finally obtain high-content sulforaphane powder. After quality testing, different specifications of the product can be prepared according to customer requirements, including standard specifications with sulforaphane content of 1%, 5%, 10%, and 13%, or customized according to customer requirements.
sulforaphane powder benefits
sulforaphane benefits for skin
Sulforaphane has garnered significant attention in the field of skin health in recent years. Studies have shown that sulforaphane can enhance the skin cells' own antioxidant defense capabilities by activating the Keap1-Nrf2 antioxidant signaling pathway, helping to regulate oxidative stress caused by aging and environmental stimuli.
Research has found that dietary supplementation with sulforaphane significantly increased the expression levels of Nrf2 and its downstream antioxidant-related genes NQO1 and HO-1 in the skin of aged mice, while simultaneously reducing reactive oxygen species (ROS) levels and the level of matrix metalloproteinase-9 (MMP-9), which is associated with skin aging. This indicates that sulforaphane powder can help maintain the oxidative balance of skin cells. Furthermore, studies have observed that sulforaphane intervention can improve skin collagen deposition and collagen structural integrity, which is beneficial for maintaining skin elasticity and structural stability.
sulforaphane benefits for brain
Although the brain accounts for only a small percentage of the body's weight, it bears a high demand for energy metabolism. Its neuronal function is highly dependent on ATP produced by mitochondria and is also more susceptible to oxidative stress and neuroinflammation. Studies have shown that sulforaphane and its precursor sulforaphane exert neuroprotective effects through various biological regulatory mechanisms, including activating cellular antioxidant defense pathways, supporting mitochondrial function, and regulating inflammatory responses.
Because sulforaphane has some blood-brain barrier permeability, it can enter the central nervous system and participate in protecting nerve cells from oxidative damage and inflammatory stimulation. Furthermore, related research indicates that sulforaphane powder may help mitigate neurological damage caused by environmental pollutants, advanced glycation end products (AGEs), and other factors, and support healthy glucose metabolism and the maintenance of brain function.
sulforaphane benefits for liver
The liver, as a vital metabolic and detoxification organ, is prone to cell damage and functional disorders when chronically exposed to oxidative stress, inflammatory responses, and metabolic burden. Studies have shown that sulforaphane, as a mild Nrf2 activator, can enhance the body's ability to resist oxidative damage by regulating the Keap1-Nrf2 signaling pathway, promoting the expression of endogenous antioxidant and cell protection-related genes.
In a sickle cell disease mouse model, dietary supplementation with sulforaphane increased the expression of Nrf2-dependent cell protection genes, improved liver function indicators, and significantly reduced the area of liver tissue necrosis. It also promoted the clearance of free heme released from erythrocyte lysis, alleviating liver damage caused by heme overload. Related research indicates that sulforaphane powder has potential application value in maintaining liver health and protecting hepatocyte function by regulating oxidative stress, inflammatory responses, and cellular defense mechanisms.
sulforaphane benefits for hair
Sulforaphane has recently attracted attention in studies on androgenetic alopecia (AGA). The occurrence of androgenetic alopecia is closely related to the effects of dihydrotestosterone (DHT) on hair follicles; excessive DHT can lead to the gradual miniaturization of hair follicles and affect the normal hair growth cycle.
Studies have found that sulforaphane can reduce blood DHT levels and mitigate its adverse effects on hair follicle growth. In animal experiments, sulforaphane powder intervention promoted hair regeneration in mice and increased the expression of genes and proteins related to 3α-hydroxysteroid dehydrogenase (3α-HSD) in the liver and hepatocytes, further validating its potential mechanism of regulating hair growth by accelerating DHT metabolism.
Reference:https://www.sciencedirect.com/science/article/abs/pii/S0955286321002370
https://pmc.ncbi.nlm.nih.gov/articles/PMC12030691/
https://www.sciencedirect.com/science/article/pii/S0301472X19309440
https://www.sciencedirect.com/science/article/abs/pii/S0006291X16302881
sulforaphane use
Sulforaphane, with its natural origin and diverse bioactivities, has become a highly sought-after plant-based active ingredient in functional foods, dietary supplements, nutritional health products, and cosmetics.
Sulforaphane Supplements
Sulforaphane is often developed as sulforaphane supplements, available in capsule, powder, and tablet forms, providing consumers with daily antioxidant and health management support. Sulforaphane tablets are also a common form of nutritional supplement on the market.
Sulforaphane Food
Sulforaphane and its precursor, sulforaphane glycosides, are also used in the development of functional foods and beverages, such as solid beverages, gel candies, and nutritional drinks, providing natural plant-based active ingredients for food formulations.
Sulforaphane cosmetic
With its antioxidant and anti-inflammatory properties, sulforaphane can be added to anti-aging, skin repair, and barrier protection skincare products to help improve skin damage caused by oxidative stress, meeting the demand for natural, effective, and safe ingredients.
sulforaphane side effects
Sulforaphane Safety
Current research indicates that sulforaphane generally has a good safety profile within the recommended dosage range. Common sulforaphane side effects are mainly related to gastrointestinal tolerance, such as mild bloating, stomach upset, nausea, or changes in bowel movements, and are mostly temporary.
Sulforaphane Dosage
Due to differences in sulforaphane content, dosage form, and extraction methods among different products, there is currently no unified standard for sulforaphane dosage. Specific dosage should be adjusted based on product specifications, intended use, and individual health conditions.
Sulforaphane Interactions
Studies show that sulforaphane may participate in metabolic regulation in the body by affecting certain metabolic enzyme systems. Therefore, individuals taking anticoagulants, chemotherapy drugs, prescription medications, or with special health needs are advised to consult a professional before long-term or high-dose supplementation.
From a regulatory and market application perspective, sulforaphane and related products have received widespread attention from the global functional food industry. In 2016, glucosinolate extract derived from broccoli seeds received GRAS (Generally Recognized As Safe) approval from the US FDA, allowing it to be used as a food ingredient. In 2017, China approved water extracts of broccoli seeds as a new food ingredient for food production and processing, providing a regulatory basis for the development and application of such plant active ingredients in the field of food nutrition.
where to buy sulforaphane?
If you are looking for a high-quality, stable supply of sulforaphane raw materials, KINTAIBIO® is a professional plant extract manufacturer that provides premium sulforaphane powder and broccoli seed extract to customers worldwide. The company uses high-quality broccoli seeds as its main raw material, ensuring stable sulforaphane content and batch-to-batch consistency. Products are available in various specifications of broccoli seed extract (1%, 10%, 13% Glucoraphanin ; 1%, 2%, 10% Sulforaphane ) for your selection, and OEM/ODM customization services are supported to meet different customer needs in product development.
As a reliable sulforaphane supplier, whether for commercial formulation development or bulk purchases of sulforaphane-related products, we can provide professional technical support, flexible customization solutions, and a continuous and stable supply capacity. Please contact us at sales@kintaibio.com for the latest product information.
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