Nigella Sativa Extract Powder

Chemical Properties
Thymoquinone (chemical formula: C₁₄H₁₂O₂) is the most characteristic bioactive component of black cumin seeds and belongs to the benzoquinone family of compounds.

• Molecular Weight: 164.20 g/mol
• Melting Point: 44-46°C
• Solubility: Soluble in organic solvents, with low solubility in water
• Stability: Sensitive to light and heat, and easily decomposes under alkaline conditions

Distribution in Raw Materials

• Natural Content: Approximately 0.5%-1.5% in black cumin seeds
• Existence: Primarily found in the volatile oil fraction of the seeds
• Influencing Factors: Content is affected by factors such as growing region, harvest time, and storage conditions.

Extraction and Enrichment
Thymoquinone can be enriched to 1%-20% through or solvent extraction combined with resin purification techniques to meet diverse application requirements.

Extraction Process
We have abandoned traditional extensive processing methods. Using the L9(3⁴) orthogonal design method, we established the optimal extraction process: 8x the volume of 60% ethanol, followed by two 1-hour extractions in a water bath under reflux. This process efficiently and evenly extracts total saponins and flavonoids, laying the foundation for high yields in subsequent purification.

• Solvent System: 60% ethanol in water
• Material-to-liquid ratio: 1:8 (raw material: solvent)
• Extraction Method: Water bath reflux extraction
• Number of extractions: 2, 1 hour each
• Temperature Control: 80±2°C

Purification Process: Using AB-8 macroporous adsorption resin, the sample solution was controlled at a pH of 5.2 and adsorbed at a flow rate of 2 BV/h. Elution was then performed using 5 BV of 70% ethanol at a flow rate of 3 BV/h.

Post-Processing Process

• Concentration: Concentration under reduced pressure, temperature ≤ 60°C
• Drying: Spray drying, inlet air temperature 180°C, outlet air temperature 80°C
• Carrier: Add ≤ 5% maltodextrin as needed

Physical and Chemical Specifications

• Appearance: Yellow-brown to brown powder
• Particle Size: 80-100 mesh
• Moisture: ≤5.0%
• Bulk Density: 0.4-0.6 g/mL
• Microbiological Specifications: Meets food ingredient standards

Active Ingredient Specifications

Thymoquinone: 1%, 5%, 10%, 20% (HPLC assay)

Comprehensive Active Ingredient Spectrum:

This product is not only a carrier of thymoquinone, but also a synergistic natural complex.
• Terpenes and Quinones: Thymoquinone (≥3%) is the core, supplemented by carvacrol and thymohydroquinone, contributing to antioxidant and anti-inflammatory activities.
• Saponins: Rich in various saponins, such as hederagenin, supporting its traditional respiratory health and immune-modulating effects.

• Flavonoids: Quercetin, Kaempferol, etc.
• Fatty Acids: Linoleic acid (45%-65%)
• Others: Tocopherols, Phytosterols, etc.

Safety Indicators
Heavy Metals: Meets USP standards
Microbial Count: ≤1000 CFU/g
Residual Solvents: Meets ICH guidelines

• Column: C18 (4.6 x 250 mm, 5 μm)
• Mobile Phase: Methanol-water gradient elution
• Detection Wavelength: 254 nm
• Column Temperature: 30°C
• Flow Rate: 1.0 mL/min

Method Validation Results:

• Linear Range: 0.04-0.24 mg (R² = 0.9991)
• Precision: RSD = 0.83%
• Repeatability: RSD = 1.31%
• Recovery: 99.60% (RSD = 1.43%)

Stability Characteristics

• Light Sensitivity: Store away from light
• Thermal Stability: Recommended storage temperature ≤ 25°C
• Humidity Sensitivity: Relative humidity ≤ 65%

Storage Conditions

• Packaging: Double polyethylene bag with aluminum foil outer bag; 25 kg/drum
• Storage: Store in a cool, dry place, away from direct sunlight
• Shelf Life: 24 months

nigella sativa extract Anti-inflammatory

The various bioactive plant compounds found in Nigella sativa seed extract work synergistically to regulate the body's inflammatory response. Clinical studies indicate that thymoquinone exerts potent anti-inflammatory and antioxidant effects by modulating oxidative stress levels, reducing the production of reactive oxygen species (ROS), inhibiting the aberrant activation of inflammatory signaling pathways (such as NF-κB), and influencing the expression of pro-inflammatory cytokines like TNF-α, IL-1β, and IL-6.

 

Furthermore, Nigella sativa seed extract can mitigate cellular oxidative damage and help maintain immune homeostasis. It holds promising potential for supporting metabolic, cardiovascular, and respiratory health in the context of chronic low-grade inflammation. In recent years, increasing international research has identified Nigella sativa seed extract as a key candidate among natural plant-based anti-inflammatory agents, with ongoing exploration into its therapeutic potential for conditions such as diabetes, cardiovascular disease, asthma, obesity, and other chronic inflammatory disorders.

nigella sativa skin benefits

Modern dermatological research indicates that Nigella sativa seed extract holds significant potential for applications in skin health and functional skincare. Extensive studies suggest that thymoquinone-a key component-helps mitigate oxidative stress-induced damage to skin cells, inhibits the release of inflammatory mediators, improves the microenvironment of the skin barrier, and supports overall skin health.

 

Given these properties, Nigella sativa seed extract has garnered widespread attention and is being utilized in the formulation of products designed for soothing and repair, sensitive skin care, acne management, anti-aging, and scalp care; it also shows promise in areas such as promoting wound healing, addressing pigmentation issues, and maintaining skin health.

nigella sativa extract Gastrointestinal

Nigella sativa seed extract holds significant research value regarding gastrointestinal health, offering support for the digestive system. Experimental studies indicate that thymoquinone helps mitigate oxidative stress-induced damage to gastrointestinal tissues and maintains the integrity of the gastrointestinal mucosal barrier. Furthermore, it plays a positive regulatory role in liver health as well as lipid and glucose metabolism, demonstrating promising potential for applications in maintaining gastrointestinal function, protecting the liver, and supporting metabolic health.

 

In recent years, Nigella sativa seed extract has been widely utilized in dietary supplements, functional foods, and botanical health products, garnering market attention as a natural, functional plant-based ingredient that supports digestive health. Notably, current research suggests it is best suited as a nutritional supplement within the context of a balanced diet and healthy lifestyle, serving as an adjunct to the comprehensive management of certain chronic conditions.

nigella sativa extract Antimicrobial

Modern research indicates that bioactive compounds within Nigella sativa seed extract possess antimicrobial properties, exerting their effects through mechanisms such as inhibiting the growth of certain pathogenic microorganisms. The extract and its essential oil demonstrate inhibitory activity against specific Gram-positive bacteria, oral pathogens, and certain drug-resistant strains; notably, thymoquinone has attracted significant research interest regarding its efficacy against drug-resistant bacteria like methicillin-resistant Staphylococcus aureus (MRSA).

 

Furthermore, experimental studies have reported the inhibitory effects of Nigella sativa seed volatile oil on intestinal parasites, providing a theoretical basis for its development in foods, dietary supplements, and natural health products. However, further high-quality clinical research is required to substantiate the clinical utility and precise mechanisms of action regarding its antimicrobial properties.

Reference:https://www.mdpi.com/2223-7747/12/22/3829

https://pmc.ncbi.nlm.nih.gov/articles/PMC12365395/

https://www.mdpi.com/1467-3045/45/4/198

https://www.mdpi.com/1420-3049/29/20/4914

Immune Modulation:
This product can modulate T lymphocyte subsets (such as CD4+/CD8+) and activate the phagocytic function of macrophages, thereby helping maintain a healthy immune response. This property makes Nigella Sativa Extract Powder ideal for use in dietary supplements designed to enhance immunity, improve allergy risk, and support respiratory health.

nigella sativa extract supplement

Nigella Sativa Extract is commonly used to produce nigella sativa extract capsules, tablets, solid beverages, oral liquids, and other nutritional supplements. Rich in thymoquinone and various natural bioactive plant compounds, it supports antioxidant defense, immune health, metabolic health, and daily nutritional management; consequently, it is widely utilized in the development of functional formulations targeting immune regulation, weight management, and the maintenance of healthy blood sugar and lipid levels.

• Recommended Dosage: 1-3 grams/day (5% thymoquinone)
• Recommended Dosage Form: Hard capsules, soft capsules, tablets
• Compatibility Considerations: Avoid direct contact with strong oxidants and alkaline substances

Nervous System Health:
Its antioxidant and anti-inflammatory properties work together on the nervous system, demonstrating potential to protect against neuronal damage in experimental models. This provides scientific support for the development of brain nutritional supplements targeting memory, cognition, and mood.

Nigella Sativa Extract For Health food

• Dosage: Determined based on product design requirements
• Process Adaptability: Excellent processing stability

Potential in Animal Nutrition:
As a natural plant-based feed ingredient, it demonstrates significant potential for enhancing livestock immunity, improving intestinal health, and reducing antibiotic use, aligning with the industry trend toward "antibiotic-free farming."

Nigella sativa extract for cosmetics

Nigella Sativa Extract is utilized in skincare formulations-such as creams, serums, masks,nigella sativa extract lotion and cleansing products-serving as a natural botanical active ingredient for soothing and repair, sensitive skin care, anti-aging, acne treatment, and scalp health. It is also suitable for the development of shampoos, conditioners, and hair growth products, helping to improve the scalp environment, maintain hair follicle health, and enhance the added value of hair care products.

• Long History of Safe Consumption: Black cumin seeds and their oil have a long tradition of consumption in many parts of the world. The U.S. FDA has designated the oil as GRAS (Generally Recognized as Safe), supporting its safety.
   
• Recommended Dosage: For powder standardized to 5%-10% thymoquinone, the recommended daily adult dietary supplement dose is between 500mg and 1500mg. The specific dosage should be determined based on the end product design.
   
• Regulatory Compliance: Our production system complies with relevant food safety production regulations in accordance with GB 16740-2014. Product specifications can be adjusted to meet the regulatory requirements of different markets (e.g., food and feed).

Compliance
Food Applications: Complies with the requirements of EU Regulation (EC) No. 1334/2008 (on food flavorings). May be labeled as "Food Flavoring Preparations" or "Black Cumin Extract."
Dietary Supplement Applications: May be labeled as "Black Cumin Extract."

Cosmetic Applications: Complies with the requirements of EU Regulation (EC) No. 1223/2009 (Cosmetics Regulation).
INCI Name: Nigella Sativa Seed Extract
CAS Number: 90064-32-7
EINECS Number: 290-094-1
Restricted Substances: The content of restricted substances such as methyl eugenol and estragole in this product strictly complies with the relevant limits set forth in the aforementioned EU regulations and IFRA standards.
REACH Status: This substance is exempt from registration according to Article 9 of Annex V to REACH Regulation (EC) No. 1907/2006.

• Quality Specifications: Conforms to the current edition of the Chinese Pharmacopoeia.