Goldenseal Root Extract

Chromatographic conditions and system suitability test: chromatographic column: ODS25u (4.6×200mm); mobile phase: acetonitrile as phase A, acetonitrile-0.01mol/ml SDS (adjust pH to 3 with phosphoric acid) (15:85) as phase B, according to A: B=30:70 elution; detection wavelength: 300nm; flow rate: 0.8ml/min. The number of theoretical plates is not less than 2200 based on berberine.
Preparation of reference solution: Accurately weigh appropriate amounts of Coptisine and Berberine from Chemicalbook, dissolve in methanol, and prepare reference solutions of 0.2 mg/ml (Coptisine) and 0.5 mg/ml (Berberine).
Preparation of the test solution: Accurately weigh an appropriate amount of Coptis chinensis extract powder, place it in a 50ml volumetric flask, add 30ml methanol, ultrasonically extract for 15 minutes, place it at room temperature, constant volume, filter with a 0.45μm microporous membrane, and set aside.
Determination method: 10 μl each of the sample test solution and the reference solution were injected into the HPLC instrument, and the contents were calculated by the area external standard method.

Fig. 1. HPLC analysis of the major benzylisoquinoline alkaloids (BIA) in goldenseal (Hydrastis canadensis). (A) Chemical structures of berberine, canadine, and hydrastine. (B) Representative of HPLC-DAD chromatogram of alkaloid standards and of whole rhizome goldenseal sample. Key: i = hydrastine, ii = canadine, iii = berberine.

The quality testing department of Kintai Bio has been cooperating with independent third-party testing laboratories, including PONY, SGS, Eurofins, NFS, etc., to meet customer needs and implement international quality standards.

Goldenseal Root Extract, also known as buttercup, is a perennial herb native to Canada and the eastern part of the United States. Its dried roots and rhizomes are used as medicine. Rhizome bright yellow, multi-noded; stem yellow, with downy and parallel irregular nodules. The botanical medicine was included in the United States Pharmacopoeia twice (1830, 1860-1926), and later included in 13 national pharmacopoeias including France.

1. Chemical composition and application overview The medicinal value of Coptis chinensis is mainly due to the high content of isoquinoline alkaloids, including hydrastine, berberine, hydrastinine, That (canadine), canadaline, and so on. In addition, it also contains papaverin, chlorogenic acid, phytosterol, resin, volatile oil, and other ingredients.

2. Coptis chinensis has the functions of antibacterial and anti-inflammatory, astringent to stop bleeding, healing, and choleretic. It is widely used in North America to eliminate mucous membrane inflammation of the respiratory tract, digestive tract, and genitourinary tract. Various preparations have been developed, and it can be used for gastroenteritis, hypochlorhydria, gastric ulcer, indigestion, upper respiratory tract infection, menorrhagia, and cervical bleeding for oral administration; it can be used for external use to treat psoriasis, hemorrhoids, thrush or trauma, and lotion can be used For excessive leucorrhea, gargles are used for gingivitis and oral ulcers, and lotions are used for conjunctivitis.

1). We are a manufacturer that complies with GMP standards. We strictly follow GMP standards from raw material selection, production, testing, and packaging.

2). Quality control: no foreign matter, no black spots, high purity, good solubility.

3). Production capacity: 100 tons/month, stable supply;
4). We have obtained FSSC22000, ISO22000, HALAL, KOSHER, HACCP and other certifications.