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1.Products Description
KINTAI is dedicated to be your best supplier in Pure L-Theanine . KINTAI produces Pure L-Theanine Powder,which has a variety of health effects, including anti-oxidation, anti-inflammation, neuroprotection, mental regulation, anti-cancer, metabolic regulation, cardiovascular protection, liver and kidney protection, immune regulation, urogenital protection and intestinal protection, etc., especially in the fight against neurodegenerative diseases, depression, etc.
Theanine has shown a good effect on the the above. Through in vivo and in vitro experiments and related human experiments, the molecular mechanisms of some health effects have also been further elucidated. It is precisely because of these health effects that theanine can be used as a functional additive or supplement in different foods, and has a good research prospect.

Green tea plants

Green tea harvesting

L-Theanine Powder 99%

L-Theanine Powder 20%
2.Where is theanine found?
L-Theanine is a water-soluble non-protein amino acid found primarily in the tea tree. It is a unique flavor ingredient with a caramel flavor that reduces the bitterness of the caffeine. As a unique secondary metabolite in tea, L-Theanine is the main source of tea flavor. L-Theanine has been shown to aid in the production of tea volatiles, which may be the main contributor to the crunchy, chestnut flavors. It can be used as one of the important indicators for evaluating the freshness of tea.
Besides, it also has various health effects such as anti-oxidation, anti-inflammation, neuroprotection, anti-cancer, anti-anxiety, metabolic regulation, cardiovascular protection, liver and kidney protection, and immune regulation. Due to its flavor and various health benefits, L-theanine has a wide range of applications, for example as a beverage ingredient or as a dietary supplement.

3.Physicochemical properties
| Chemical formula: | C7H14N2O3 | Molecular weight: | 174.198 |
| CAS registration number: | 3081-61-6 | Melting point: | 207 ℃ |
| Boiling point: | 427.13 ℃ | Water solubility: | soluble in water, insoluble in ethanol and ether |
| Flash point: : | 212.12 ℃ | Appearance: | brown to white crystalline powder |
4.Product quality specification & standard
|
Product name |
Pure L-Theanine Powder |
|
Extract source |
Green Tea leaf |
|
Extraction Solvent |
Water/Ethyl alcohol |
|
Appearance |
Brown to white crystalline powder |
|
Solubility |
soluble in water, insoluble in ethanol and ether |
|
Identification |
TLC , HPLC |
|
Sulphated Ash |
NMT 0.5% |
|
Heavy metals |
NMT 20 PPM |
|
Loss On Drying |
NMT 5.0% |
|
Powder size |
80Mesh, NLT90% |
|
Assay (HPLC test, percent, Standard in House) |
Min. 98.0% |
|
Microbiological Quality (Total viable aerobic count) |
|
|
- Bacteria, CFU/g, not more than |
NMT 103 |
|
- Moulds and yeasts, CFU/g, not more than |
NMT 102 |
|
- E.coli, Salmonella, S. aureus, CFU/g |
Absence |
|
Shelf life |
Powder storage at room temperature 24 months |
5.KINTAI‘s Production Process Flow

6. Benefits and Application
Benefits
★Hypotensive
★Anticoagulant
★Lowering blood fat and obesity
★Prevention and treatment of cardiovascular diseases such as arteriosclerosis and thrombosis
★Hypoglycemic
★Preventing diabetes
★Bactericidal and antiviral
★Prevention and treatment of gastrointestinal, respiratory, influenza and other diseases
★Preventing hepatitis, anti-aging and enhance immune function
★Healthy teeth, prevention of periodontitis, prevention of dental caries, deodorization and detoxification, etc.

Application
(1)Medical care: health food for relieving fatigue, lowering blood pressure and improving learning and memory.
(2)Tea drink quality improver: Due to the limitation of raw materials and processing technology, tea drinks in China have poor fresh taste. Theanine is the main component of tea fresh taste, and can alleviate the bitter taste of caffeine and tea polyphenols.
(3)Functional food additive: theanine can enhance brain α Wave strength can make people feel relaxed and improve their memory.
(4)Theanine flavor improvement effect: Theanine can not only be used as a green tea flavor modifier, but also inhibit the bitterness and astringency in other foods to improve food flavor.

7.A method for extracting theanine
(1) A method for extracting theanine, comprising the steps of:
▲Step A, ultrafiltration: Ultrafiltration of waste liquid after extracting tea polyphenols from tea leaves to remove macromolecular substances in the waste liquid, said macromolecular substances including proteins and/or polysaccharides;
▲Step B, precipitation: adding basic copper carbonate to the filtrate obtained by ultrafiltration in step A, the reaction generates theanine-basic copper carbonate precipitation, centrifugation and discarding the liquid to obtain theanine-basic copper carbonate solid;
▲Step C, dissolving: the solid obtained in step B is dissolved using 0.1~2mol/L dilute sulfuric acid to generate theanine-copper sulfate mixed solution;
▲Step D, electrodialysis: the mixed solution described in step C removes the inorganic salt wherein through electrodialysis, and described inorganic salt comprises copper sulfate;
▲Step E, concentration under reduced pressure: the liquid in the fresh water chamber in step D is concentrated under reduced pressure;
▲Step F, adding ethanol to remove sulfuric acid: add the ethanol whose weight is 3 to 100 times and the concentration is 98 to 100% to the concentrated solution obtained in step E, and remove sulfuric acid with the liquid through solid-liquid separation to obtain solid theanine.

(2) According to the described method of claim 1, it is characterized in that, the ultrafiltration membrane used in the step A is ceramic membrane, and membrane aperture is 50nm, and ultrafiltration pressure is 0.15~0.5MPa.
(3) According to the described method of claim 1, it is characterized in that, the consumption of the basic copper carbonate used in step B is the 1~5wt% of step A gained filtrate.
(4) Method according to claim 1, is characterized in that, also comprises the step of using deionized water to wash described theanine-basic copper carbonate solid between step B and step C, and deionized water consumption is described tea 2~5 times of solid weight of acid-basic copper carbonate.
(5) Method according to claim 1, is characterized in that, the consumption of dilute sulfuric acid is 5~8 times of described solid weight in the step C, and the concentration of dilute sulfuric acid is 0.5~1.3mol/L in the step C.
(6) The method according to claim 1, characterized in that, electrodialysis in step D until the inorganic salt content in the liquid in the fresh water chamber is less than or equal to 20 mg/L.
(7) According to the described method of claim 1, it is characterized in that, in step E, the density that is concentrated to concentrated solution under reduced pressure is 1.05 ~ 1.25g/cm .

(8) According to the described method of claim 1, it is characterized in that, the consumption of ethanol is 8 ~ 15 times of described concentrate weight in the step F, and described solid-liquid separation is suction filtration, and gained filter cake is solid-state theanine; Preferably, the ethanol solution containing sulfuric acid obtained after the solid-liquid separation in step F is recovered and separated under reduced pressure, the obtained ethanol is reused in step F, and the obtained sulfuric acid is recycled in step C.
(9) The method according to claim 1, characterized in that step G is also included after step F, that is, flushing the solid-state theanine with 95 to 98% ethanol to remove residual sulfuric acid therein, and then drying to obtain tea Acid crude product; The concentration of ethanol for flushing is preferably 96~97.5%.
(10) According to the method described in any one in claim 1~9, it is characterized in that, the theanine content is 1~5%, preferably 1.5~5% in the waste liquid after the raw material used in the step A namely tealeaves extracts tea polyphenols 3%.
8.What can KINTAI do?


9.KINTAI'S PROCESSING

10.Why choose Kintai?

11.Kintai manufacturing base

12.Kintai R&D center

13.Package

TIPS
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