Citicoline CDP Choline Powder

Bacillus subtilis is inoculated into a culture medium containing 0.1–5% glucose, 0.5–5% beef extract, 1–10% peptone, 0.5–5% yeast extract, and 0.5–1% sodium chloride. The culture is incubated at a constant temperature of 32°C until the glucose is depleted, thereby yielding the activated strain. Subsequently, this activated culture is transferred into a seed culture medium at an inoculation ratio of 5 wt%. The seed culture medium consists of an aqueous solution containing carbon sources, nitrogen sources, and trace elements, supplemented with 0.1–3% phosphate. The mixture is cultivated under shaking or aeration conditions at 30–35°C for 5–25 hours to obtain a culture broth containing a high density of microorganism A, which is then utilized for the subsequent biotransformation process.

Choline feedstock is added to the aforementioned culture broth containing microorganism A at a concentration of 0.5–3 wt% relative to the total weight of the culture broth. Optionally, 0.005–0.05 wt% of the surfactant Tween-80 may be added to facilitate contact between the substrate and the microbial cells. The reaction temperature is maintained between 20°C and 38°C for a duration of 10–72 hours, while acid-base regulators are employed to maintain the pH within the range of 6 to 7. Under these conditions, the microorganisms utilize their intrinsic de novo pyrimidine biosynthesis pathway to accumulate and produce CDP-choline, using choline as a precursor. Upon completion of the reaction, the concentration of CDP-choline in the fermentation broth reaches a high level; the progress of the reaction is monitored using High-Performance Liquid Chromatography (HPLC).

Following the completion of fermentation, the microbial cells and cellular debris are first removed via tubular centrifugation or microfiltration to collect the clarified fermentation supernatant. The supernatant is then passed through a cation-exchange resin column to adsorb the CDP-choline. Impurities are subsequently washed away using deionized water, followed by gradient elution using a dilute ammonia solution or a sodium chloride solution to collect the eluate containing the CDP-choline. The resulting eluate undergoes decolorization treatment using activated carbon, followed by concentration under reduced pressure to remove water, thereby yielding a concentrated product solution. Anhydrous ethanol or acetone is added to the concentrate to induce crystallization, and the mixture is allowed to stand to precipitate white crystals. After centrifugation, the crystals are washed with a small amount of ethanol and finally vacuum-dried at 40–50°C to constant weight; the resulting material is then pulverized and sieved to yield Citicoline CDP-Choline powder.

In the development of dietary supplements, Citicoline CDP-Choline powder offers versatile processing capabilities, allowing it to be formulated into various dosage forms-including standard citicoline and CDP-choline supplements. Specifically, it can be manufactured as cdp choline capsules, tablets, and other formats suitable for daily cognitive maintenance. CDP-choline sodium is highly regarded for its potential cognitive-enhancing properties and its benefits for overall brain health. Such supplements are frequently promoted as aids for improving memory, focus, attention, and general cognitive function.

Citicoline (CDP-Choline) powder can also be incorporated into functional beverages to develop citicoline energy drinks, thereby meeting the immediate needs of students and working professionals for enhanced focus and mental clarity. A clinical study has confirmed that supplementing with 500 mg of citicoline daily for two consecutive weeks can significantly improve psychomotor vigilance and visual working memory.

Furthermore, given its unique role in cell membrane repair and neurotransmission, Citicoline also demonstrates potential for application in the field of ophthalmology, where it can be formulated into eye drops to help support optic nerve health. In summary, whether the objective is to enhance memory, concentration, and overall cognitive function, or to maintain brain health in middle-aged and elderly populations, the Citicoline CDP-Choline powder produced by KINTAIBIO^® offers a scientifically sound and versatile raw material solution.